|Western Blot (WB)||1:500-1:2000|
|Tested Species reactivity||Mouse, Rat, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthesized peptide derived from human NPM around the phosphorylation site of Threonine 199|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.5, with 50% glycerol|
|Contains||0.02% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
Concentration is lot-specific and will vary from 0.5-0.6 mg/ml
Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double- stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: NPM; NPM1; nucleolar phosphoprotein B23; nucleolar protein B23.1; nucleolar protein NO38; Nucleophosmin; nucleophosmin 1; nucleophosmin/nucleoplasmin family, member 1; numatrin
Gene Aliases: B23; B23NP; NO38; NPM
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