|Western Blot (WB)||1:1,000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human PAG3 that contains tyrosine 724.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
ASAP2 is a multidomain protein belonging to the beta family with two ANK repeats, an Arf-GAP domain, a PH domain and a SH3 domain. This protein localizes in the Golgi apparatus and also at the plasma membrane where it colocalizes with protein tyrosine kinase 2-beta (PYK2) and forms a stable complex with PYK2 in vivo. ASAP2 is tyrosine phosphorylated by activated PYK2. It functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. ASAP2 activates the small GTPases ARF1, ARF5 and ARF6 and regulates the formation of post-Golgi vesicles and modulates constitutive secretion. It modulates phagocytosis mediated by Fc gamma receptor and ARF6. Usually detected in heart, brain, placenta, kidney, monocytes and pancreas, it is up-regulated during monocyte maturation. Multiple transcript variants encoding different isoforms have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AMAP2; ANK repeat and PH domain-containing protein 2; arf-GAP with SH3 domain; Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2; beta 3; centaurin; centaurin, beta 3; CENTB3; DDEF2; development and differentiation enhancing factor 2; Development and differentiation-enhancing factor 2; PAG3; PAP; Pap-alpha; paxillin-associated protein w; Paxillin-associated protein with ARF GAP activity 3; PYK2 C terminus-associated protein; Pyk2 C-terminus-associated protein; SHAG1
Gene Aliases: AMAP2; ASAP2; CENTB3; DDEF2; KIAA0400; PAG3; PAP; Pap-alpha; SHAG1
UniProt ID: (Human) O43150
Entrez Gene ID: (Human) 8853
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