|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic phosphopeptide derived from human PKC-pan around the phosphorylation site of Thr497 (T-S-TP-F-C)|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.4, with 50% glycerol, 150mM NaCl|
|Contains||0.02% sodium azide|
This antibody detects PRKCA, PRKCB, PRKCD, PRKCE, PRKCG, PRKCH, PRKCQ and PRKCZ
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms ( alpha, betaI, betaII and gamma) and novel (n) PKC isoforms ( delta, epsilon, zeta, eta and theta). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and epsilon are independent of Ca2+.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 14 - 3 - 3 - zeta isoform; aging-associated gene 6; atypical protein kinase C; nPKC-delta; nPKC-epsilon; nPKC-eta; nPKC-theta; nPKC-zeta; PKC theta; PKC zeta; PKC-A; PKC-alpha; PKC-B; PKC-beta; PKC-gamma; PKC-L; PKCA; PKCB; PKCE; PKCG; PRKACA; PRKCA; PRKCB; PRKCB1; PRKCD; PRKCE; PRKCG; PRKCQ; PRKCT; PRKCZ; Protein kinase C alpha type; protein kinase C beta I; protein kinase C beta II; Protein kinase C beta type; protein kinase C beta-II; Protein kinase C delta type; protein kinase C delta variant IX; protein kinase C delta VIII; Protein kinase C epsilon type; Protein kinase C eta type; Protein kinase C gamma type; Protein kinase C theta type; protein kinase C type I (gamma type); protein kinase C zeta subspecies; Protein kinase C zeta type; protein kinase C, alpha; protein kinase C, beta 1; protein kinase C, beta 1 polypeptide; protein kinase C, delta; protein kinase C, delta IV; protein kinase C, delta V; protein kinase C, epsilon; protein kinase C, eta; protein kinase C, gamma; protein kinase C, theta; protein kinase C, zeta; protein kinase c-alpha; protein kinase C-eta; protein kinase C[d]; SDK1; Sphingosine-dependent protein kinase-1; Tyrosine-protein kinase PRKCD
Gene Aliases: 14-3-3-zetaisoform; 5830406C15Rik; A130035A12Rik; A130082F03Rik; AAG6; AI098070; AI385711; AI875142; ALPS3; aPKCzeta; AW494342; C80388; CVID9; D14Ertd420e; MAY1; nPKC-delta; nPKC-epsilon; nPKC-eta; nPKC-theta; nPKC-zeta; PKC; PKC-0; PKC-alpha; PKC-Beta; PKC-gamma; PKC-L; PKC-theta; PKC-ZETA; PKC2; PKC[d]; PKC[e]; PKCA; PKCB; PKCC; PKCD; PKCdelta; PKCE; Pkcea; PKCepsilon; PKCG; PKCgamma; Pkch; PKCI; PKCL; Pkcq; PKCtheta; Pkcz; PRKACA; Prkc; PRKCA; PRKCB; PRKCB1; PRKCB2; Prkcc; PRKCD; PRKCE; PRKCG; PRKCH; PRKCL; PRKCQ; PRKCT; PRKCZ; r14-3-3; R74924; R75156; RATPKCI; SCA14; zetaPKC
UniProt ID: (Human) P17252, (Human) P05771, (Human) Q05655, (Human) Q02156, (Human) P05129, (Human) P24723, (Human) Q04759, (Human) Q05513, (Mouse) P20444, (Mouse) P68404, (Rat) P68403, (Rat) P09215, (Mouse) P28867, (Mouse) P16054, (Rat) P09216, (Rat) P63319, (Mouse) P63318, (Mouse) P23298, (Rat) Q64617, (Mouse) Q02111, (Rat) P09217, (Mouse) Q02956
Entrez Gene ID: (Human) 5578, (Human) 5579, (Human) 5580, (Human) 5581, (Human) 5582, (Human) 5583, (Human) 5588, (Human) 5590, (Rat) 24680, (Mouse) 18750, (Mouse) 18751, (Rat) 25023, (Rat) 170538, (Mouse) 18753, (Mouse) 18754, (Rat) 29340, (Rat) 24681, (Mouse) 18752, (Mouse) 18755, (Rat) 81749, (Rat) 85420, (Mouse) 18761, (Rat) 25522, (Mouse) 18762
Molecular Function: annexin calcium-binding protein calmodulin intracellular calcium-sensing protein kinase non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.