|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide sequence around phosphorylation site of serine 676 (R-L-S(p)-F-A) derived from Human PKC-theta|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
A suggested positive control for Western blot is PC12 cells; suggested positive control for IHC is human lung carcinoma; suggested positive control for ICC/IF is Hela cells.
Members of the Protein kinase C (PKC) family of serine/threonine protein kinases are grouped by their activation mechanism. Classical or conventional PKCs (PKC alpha-, betaI- , betaII- and gamma-) are activated by phosphatidylserine in a calcium dependent manner and can bind diacylglycerol. The Ca2+ insensitive novel PKCs (PKCs epsilon-, delta-, theta- and eta) are also activated by diacylglycerol and phosphatidylserine. The atypical PKCs (PKCs iota- and zeta-) are insensitive to Ca2+, DAG and phorbolesters. All PKCs isoforms consist of a highly conserved catalytic domain connected to a regulatory domain via a hinge region. PKCs are involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascades involving MAPK1/3 (ERK1/2) and RAP1GAP.
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Protein Aliases: EC 184.108.40.206; kinase PKC-theta; KPCT; nPKC-theta; PKC theta; PKC-theta; PKCQ; PRKCQ; PRKCT; Protein kinase C theta type; protein kinase C, theta; theta type
Gene Aliases: A130035A12Rik; AW494342; nPKC-theta; PKC-0; PKC-theta; Pkcq; PKCtheta; PRKCQ; PRKCT
Molecular Function: annexin calcium-binding protein calmodulin intracellular calcium-sensing protein kinase non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase
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