|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Western Blot (WB)||1:100-1:1000|
|Western Blot (WB)||See 2 publications below|
|Gel Shift (GS)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Human Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues I(8) C P L (pS) P L E A D D L(19) of mouse PPAR alpha.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-820 detects phospho-peroxisome proliferator activated receptor (PPAR) alpha S12 from mouse tissues.
PA1-820 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~52 kDa protein which corresponds to phospho-PPAR alpha S12 from mouse adipose tissue extract.
The PA1-820 immunogen is a synthetic phosphopeptide corresponding to residues I(8) C P L (pS) P L E A D D L(19) of mouse PPAR alpha. This peptide (Cat. # PEP-181) is available for use in neutralization and control experiments.
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPAR and quote;s). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPAR and quote;s are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR and quote;s can induce transcription of acyl coenzyme A oxidase and cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Nuclear receptor subfamily 1 group C member 1; peroxisome proliferative activated receptor, alpha; Peroxisome Proliferator Activated Receptor Alpha; peroxisome proliferator-activated nuclear receptor alpha variant 3; Peroxisome proliferator-activated receptor alpha; PPAR-alpha
Gene Aliases: 4933429D07Rik; AW742785; hPPAR; NR1C1; PPAR; PPAR-alpha; PPARA; PPARalpha
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.