|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||1:1000|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic phospho peptide derived from a region of the human Progesterone Receptor (hPR) protein surrounding the phosphorylated Ser190 residue.|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 100µg/ml BSA|
37-8200 detects Phospho-Progesterone Receptor in Human samples.
37-8200 has been successfully used in western blot, immunofluorescence and immunohistochemistry in human samples. Phospho-Progesterone Receptor (Ser 190) antibody detects both progesterone receptor A and B forms when phosphorylated at Ser26 and Ser190, respectively.
The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: NR3C3; Nuclear receptor subfamily 3 group C member 3; PR; Progesterone receptor
Gene Aliases: NR3C3; PGR; PR
UniProt ID: (Human) P06401
Entrez Gene ID: (Human) 5241
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