|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 ug/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||4 ug/ml|
|Immunocytochemistry (ICC)||See 1 publications below|
|Western Blot (WB)||See 2 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Mouse Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic phosphopeptide corresponding to residues V(184) L P R G L S(p) P A R Q L L(196) of human Progesterone Receptor.|
|Storage buffer||1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-413 detects phospho-progesterone receptor (pSer190) from human samples.
MA1-413 has been successfully used in Western blot, immunohistochemistry, ELISA, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody detects ~81 and ~116 kDa proteins representing phospho-progesterone receptor (pSer190) A and B from T47D cell and human breast carcinoma extracts. Immunohistochemical staining of phospho-progesterone receptor (pSer190) (paraffin samples) can be performed using MA1-413.
The MA1-413 immunogen is a synthetic peptide corresponding to residues V(184) L P R G L S(p) P A R Q L L(196) of human Progesterone Receptor.
The progesterone receptor is a member of the Steroid Hormone Receptor Superfamily. It exists in two distinct isoforms in human cells. PR-B is the transcriptionally active form and is responsible for activating genes for the maintainence of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. Phospholabeling studies have shown that PR is phosphorylated in at least 9 different locations in response to progestins, which suggests that PR affects many different cellular pathways.
Ser 190 is one of four sites that demonstrate basal phosphorylation in the absence of ligand. The level of phosphorylation rapidly increases with addition of receptor agonists. This antibody recognizes the phosphorylated form of both PR-A and PR-B at Ser 190.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: NR3C3; Nuclear receptor subfamily 3 group C member 3; PR; Progesterone receptor
Gene Aliases: NR3C3; PGR; PR
UniProt ID: (Human) P06401
Entrez Gene ID: (Human) 5241
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