|ChIP assay (ChIP)||1 - 3µl|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25|
|Western Blot (WB)||1:1000|
|Tested Species reactivity||Hamster, Human, Mouse, Rat, Yeast|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pSer2/5 of the Rpb1 CTD heptapeptide|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
It is not recommended to aliquot this antibody.
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DNA polymerase epsilon catalytic subunit A; DNA polymerase II subunit A; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; Pol2p; polymerase (DNA directed), alpha 1, catalytic subunit; Polymerase (RNA II (DNA directed), large polypeptide; polymerase (RNA) II (DNA directed) polypeptide A; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; RNA polymerase II 1; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1
Gene Aliases: 220kDa; DUN2; hRPB220; hsRPB1; I79_016081; N0825; POL2; POLR2; POLR2A; POLRA; RPB1; RPBh1; Rpii215; RpIILS; RPO2; Rpo2-1; RPOL2; YNL262W
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