|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunocytochemistry (ICC)||1 µg/ml|
|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:500|
|Western Blot (WB)||1-2 µg/mL|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunoprecipitation (IP)||See 3 publications below|
|Western Blot (WB)||See 4 publications below|
|Tested Species reactivity||Chicken, Human, Mouse, Rat|
|Published species reactivity||Rat , Bovine , Human Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Src that contains tyrosine 529 (tyrosine 530 including the initiating methionine). The sequence is conserved in mouse, rat, chicken and frog.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. Transcript Variant: This variant (1) represents the longer transcript. Both isoforms 1 and 2 encode the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: c-Src; EC 18.104.22.168; Neuronal proto-oncogene tyrosine-protein kinase Src; non-tyrosine protein kinase; p60-Src; pp60c-src; Proto-oncogene c-Src; Proto-oncogene tyrosine-protein kinase Src; protooncogene SRC, Rous sarcoma; Rous sarcoma oncogene; tyrosine kinase pp60c-src; tyrosine protein kinase c-src; tyrosine protein kinase pp60-c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
Gene Aliases: ASV; AW259666; c-SRC; p60-Src; PP60C-SCR; pp60c-src; SDR; SRC; SRC1; THC6
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