|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide sequence around phosphorylation site of tyrosine 323 (N-P-Y(p)-E- P) derived from Human syk.|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
A suggested positive control for Western blot is JK cells; suggested positive control for IHC is human breast carcinoma; suggested positive control for ICC/IF is Hela cells.
SYK is a positive effector of BCR stimulated responses. It couples the B cell antigen receptor (BCR) to the mobilization of calcium ions either through a phosphoinositide 3 kinase dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C gamma dependent pathway, when phosphorylated on Tyr 342 and Tyr 346. Thus the differential phosphorylation of SYK can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ions.
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Protein Aliases: EC 184.108.40.206; kinase Syk; KSYK; p72-Syk; Spleen tyrosine kinase; SYK; Tyrosine-protein kinase SYK
Gene Aliases: p72-Syk; SYK
UniProt ID: (Human) P43405
Entrez Gene ID: (Human) 6850
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