|Western Blot (WB)||1:1,000|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser603 of rat SYN1 conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This antibody is predicted to react with human, Xenopus, bovine, mouse and zebrafish based on 100% sequence homology.
In Western blot, this antibody detects an ~78 kDa protein representing Phospho-Ser603 Synapsin I protein.
Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of Synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus Synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis.
The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Serine603 is the site on Synapsin I that is phosphorylated by calcium calmodulin kinase II and by p21-activated kinases (Sakurada et al., 2002; Czernik et al., 1987). Phosphorylation of this site is thought to regulate synaptic vesicle function (Nayak et al., 1996; Bahler and Greengard, 1987; McGuinness et al., 1989).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: synapsin 1; Synapsin I; Synapsin-1
Gene Aliases: Syn1
UniProt ID: (Rat) P09951
Entrez Gene ID: (Rat) 24949
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