|Western Blot (WB)||Assay-Dependent|
|Immunohistochemistry (IHC)||See 4 publications below|
|Western Blot (WB)||See 2 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Mouse Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from a region of human Tau that contains threonine 231.|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
OPA1-03156 detects human p-Tau (pThr231) from human samples. Mouse and rat Tau have not been tested but are expected to react based on sequence homology.
OPA1-03156 has been successfully used in Western blot procedures.
OPA1-03156 immunizing phosphopeptide was derived from a region of human tau that contains threonine 231.
Tau is a neuronal microtubule-associated protein found predominantly on axons and functions to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer and quote;s disease (AD) brain. Hyperphosphorylated Tau is also found in neurofibrillary lesions in a range of other central nervous system disorders. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases including GSK-3ß, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II (CK2), phosphorylate Tau. Threonine 231 is phosphorylated by GSK-3ß, cdk5 and cdk1, and has been shown to be involved in the pre-tangle process in AD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DDPAC; Disinhibition Dementia Parkinsonism Amyotrophy Complex; FLJ31424; FTDP17; G protein beta1/gamma2 subunit-interacting factor 1; MAPTL; Microtubule Associated Protein Tau; Microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; MSTD; Neurofibrillary Tang; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau; protein phosphatase 1, regulatory subunit 103
Gene Aliases: DDPAC; FTDP-17; MAPT; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; TAU
UniProt ID: (Human) P10636
Entrez Gene ID: (Human) 4137
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