|Western Blot (WB)||1:1000|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser260 of rat TPH1 conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This antibody is predicted to react with bovine, canine, chicken, mouse and zebrafish based on 100% sequence homology.
This antibody is specific for the ~55 kDa Tryptophan Hydroxylase protein phosphorylated at Ser260 in Western blots of human dorsal Raphe nucleus.
Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: indoleacetic acid-5-hydroxylase; L-tryptophan hydroxylase; TPH; TPH1; TPRH; Tryptophan 5 hydroxylase 1; Tryptophan 5 monooxygenase 1; Tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; tryptophan hydroxylase (tryptophan 5-monooxygenase); Tryptophan hydroxylase 1
Gene Aliases: TPH; TPH1; TPRH; TRPH
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.