|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||2 ug/ml|
|Immunofluorescence (IF)||2 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||2-3 ug/ml|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Phosphoeptide corresponding to amino acids 935–944 of human TSC2 [pS939]|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with chimpanzee, equine and non-human primate based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Tuberin (TSC2) is a tumor suppressor protein which forms a heterodimer with hamartin involved in regulation of cell growth and size. It acts to inhibit mTOR, resulting in the downstream activation of 4E-BP1, which leads to inhibition of translation. It is also involved in microtubule mediated protein transport. Tuberin expression is present in most tissues, and is co-localized with hamartin. Phosphorylation of tuberin on Ser 939 and Thr 1462 regulates its interaction with hamartin, and is stimulated by various growth factors through the phosphoinositide 3-kinase/Akt pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: protein phosphatase 1, regulatory subunit 160; TSC4; Tuberin; Tuberous sclerosis 2 protein; tuberous sclerosis 2 protein homolog
Gene Aliases: LAM; Nafld; PPP1R160; Tcs2; TSC2; TSC4
UniProt ID: (Human) P49815
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.