|Immunofluorescence (IF)||8 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||8 ug/ml|
|Western Blot (WB)||0.5 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunoprecipitation (IP)||See 2 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Bovine, Human, Rat|
|Published species reactivity||Rat Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues F(54) L R A P S(p) W I D T G(64) of human alpha-B Crystallin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 30mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-012 detects phospho-alpha-B crystallin (Ser59) from bovine, human and rat samples. This antibody does not detect the unphosphorylated form of the protein.
PA1-012 has been successfully used in Western blot, immunohistochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~20 kDa protein representing recombinant bovine phospho-alpha-B crystallin (Ser59). Immunofluorescence staining of phospho-alpha-B crystallin (Ser59) in human brain from Alexander and quote;s diseased patients results in staining of cells in the cerebral cortex.
The PA1-012 immunogen is a synthetic phosphopeptide corresponding to residues F(54) L R A P S(p) W I D T G(64) of human alpha-B Crystallin.
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens.
Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander and quote;s disease where it accumulates in brain cells of those afflicted.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Alpha(B)-crystallin; Alpha-crystallin B chain; CRYA2; crystallin, alpha B; crystallin, alpha polypeptide 2; CTPP2; epididymis secretory protein Li 101; Heat shock protein beta-5; heat-shock 20 kD like-protein; HSPB5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component
Gene Aliases: AACRYA; BOS_14343; CMD1II; CRYA2; CRYAB; CTPP2; CTRCT16; HEL-S-101; HSPB5; MFM2
Molecular Function: chaperone
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