|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide sequence around phosphorylation site of threonine 239 (G-E-T(p)-P-P) derived from Human c-Jun.|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
A suggested positive control for Western blot is Hela cells; suggested positive control for IHC is human breast carcinoma; suggested positive control for ICC/IF is Hela cells.
Fos and Jun dimerize to form Activator Protein-1 (AP-1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13-acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the and quote;leucine-zipper and quote; motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP-1 dependent genes.
Protein Aliases: Activator protein 1; AH119; AP1; Avian sarcoma virus 17 (v-jun) oncogene homolog; c-Jun; enhancer-binding protein AP1; immediate early; Jun A; Jun activation domain binding protein; Jun oncogene; jun proto-oncogene; p39; Proto-oncogene c-Jun; RJG-9; Transcription factor AP-1; V-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog
Gene Aliases: AP-1; AP1; c-Jun; JUN; Junc; Rjg-9