|Western Blot (WB)||1:1,000|
|Tested Species reactivity||Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser289/296/301 of human c-Raf.|
|Storage buffer||0.01M HEPES, pH 7.5, with 100µg/ml BSA, 50% glycerol, 0.15M NaCl|
Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cRaf; Craf1 transforming; murine sarcoma 3611 oncogene 1; Oncogene RAF1; proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; sarcoma 3611 oncogene; v-raf-1 leukemia viral oncogene 1
Gene Aliases: 6430402F14Rik; AA990557; BB129353; c-Raf; CRAF; Craf1; D830050J10Rik; NS5; Raf-1; v-Raf
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