|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||1:1000|
|Western Blot (WB)||See 2 publications below|
|Tested Species reactivity||Fruit fly, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pSer51 of human eIF2alpha|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
It is not recommended to aliquot this antibody.
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CG9946-PA; CG9946-PB; eIF-2-alpha; EIF-2A; EIF-2alpha; eIF-2alpha-PA; eIF-2alpha-PB; eukaryotic initiation factor 2alpha; eukaryotic translation initiation factor 2; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; eukaryotic translation initiation factor 2A; eukaryotic translation initiation factor 2alpha; minute (1) 14C; OTTHUMP00000218025
Gene Aliases: 0910001O23Rik; 2410026C18Rik; 35kDa; CG9946; deIF2alpha; Dmel\CG9946; Dmel_CG9946; EIF-2; eIF-2 a; eIF-2 alpha; EIF-2A; EIF-2alpha; eIF-2alphaeIF-2alpha; EIF2; eIF2 alpha; eIF2-alpha; EIF2A; Eif2alpha; EIF2S1; elF2alpha; IF2A_DROME; l(1)14Cf; l(1)19-153; M(1)14C; M(1)19-153; M(1)8e3-10
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