|Western Blot (WB)||1:5000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospholyrated peptide (KLH coupled) corresponding to residues around Thr180/Tyr182 of human p38 MAP kinase.|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
A suggested positive control for this product is Jurkat cells treated with anisomycin.
p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation and other signals. p38 MAPK is relatively inactive in the non-phosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, a, b, g and d, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor-a, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 and-6 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CRK1; CSAID-binding protein; Csaids binding protein; cytokine suppressive anti-inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug binding protein 1; Cytokine suppressive anti-inflammatory drug-binding protein; cytokine-supressive anti-inflammatory drug binding protein; MAP kinase 14; MAP kinase 2; MAP kinase MXI2; MAP kinase p38 alpha; MAPK 14; MAX-interacting protein 2; mitogen activated protein kinase 14; Mitogen-activated protein kinase 14; mitogen-activated protein kinase 14A; Mitogen-activated protein kinase p38; Mitogen-activated protein kinase p38 alpha; p38 alpha; p38 MAP kinase; p38 MAP kinase alpha; p38 MAPK; p38 mitogen activated protein kinase; p38alpha Exip; reactive kinase; SAPK2a; Stress-activated protein kinase 2a; tRNA synthetase cofactor p38
Gene Aliases: CRK1; CSBP; CSBP1; CSBP2; CSPB1; EXIP; Hog; MAPK14; MXI2; p38; p38-alpha; p38a; p38ALPHA; p38Hog; p38MAPK; PRKM14; PRKM15; RK; SAPK2A
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