|Flow Cytometry (Flow)||1:20|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500|
|Western Blot (WB)||1:250|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Western Blot (WB)||See 5 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Human, Rat|
|Published species reactivity||Rat , Human Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to the region surrounding the phosphorylated Thr202 and Tyr204 residues of ERK1+2 (p44/42 MAP kinase).|
|Storage buffer||0.01M HEPES, pH 7.5, with 50% glycerol, 0.15M NaCl, 100µg/ml BSA|
This antibody is predicted to react with Xenopus and zebrafish based on 100% sequence homology.
36-8800 is specific for the ~42k - 44k ERK/MAPK phosphorylated at Thr202 and Tyr204. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by lambda-phosphatase.
Extracellular signal-regulated kinase 2 (Erk2) is also known as mitogen activated protein kinase 2, microtubule-associated protein-2 kinase MAP kinase 2, p42-MAPK or EGF receptor T669 (ERT1). Mek2 activates Erk2 by phosphorylation of neighbouring threonine-183 and tyrosine-185 residues. Erk2, a serine-threonine kinase phosphorylates microtubule associated protein-2 and myelin basic protein. This kinase is an important proximal componant of the MAP kinase pathway involved in transmitting the signals from growth factors, neurotransmitters and hormones at the cell surface to the transcriptional events in the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; MAPK1; MAPK3; Microtubule-associated protein 2 kinase; mitogen-activated 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; MNK1; p42-MAPK; p44-ERK1; p44-MAPK; PRKM1; PRKM2; PRKM3; protein tyrosine kinase ERK2
Gene Aliases: ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; Mapk; MAPK1; MAPK2; MAPK3; MNK1; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3
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