|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||1 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||KLH-conjugated phosphopeptide RHKKLMFKTEGPDS[P]D, corresponding to amino acids 378-393 of human p53.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage conditions||4° C, do not freeze|
For IHC staining, high temperature antigen retrieval (microwave, pressure cooker) is recommended, using 10 mM citrate buffer, pH 6.0, or 1 mM EDTA-NaOH buffer, pH 8.0.
The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.
Protein Aliases: Antigen NY-CO-13; Cellular tumor antigen p53; Cys 51 Stop; HGNC11998; mutant tumor protein 53; p53 Cellular Tumor Antigen; p53 Tumor Suppressor; Phosphoprotein p53; transformation-related protein 53; TRP53; tumor protein 53; Tumor suppressor p53; tumor supressor p53; Tumour Protein p53
Gene Aliases: BCC7; LFS1; P53; TP53; TRP53
UniProt ID: (Human) P04637
Entrez Gene ID: (Human) 7157
Molecular Function: transcription factor