|Western Blot (WB)||1:500-1:2000|
|Tested Species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from human p95/NBS1 around the phosphorylation site of Serine 343|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
Concentration is lot-specific and will vary from 0.5-0.6 mg/ml
Nibrin is a component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. NBN modulates the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. NBS1 forms a complex with RBBP8 to link DNA double-strand break sensing to resection. It enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cell cycle regulatory protein P95; NBN; NBS; NIBRIN; Nijmegen breakage syndrome 1 (nibrin); NIJMEGEN BREAKAGE syndrome protein 1; nijmegen breakage syndrome protein 1 homolog; p95 protein of the MRE11/RAD50 complex; p95-NBS1
Gene Aliases: AT-V1; AT-V2; ATV; NBS; NBS1; P95
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