|ELISA (ELISA)||1 µg/ml|
|Flow Cytometry (Flow)||Assay dependent|
|Immunocytochemistry (ICC)||1:50 - 1:200|
|Immunofluorescence (IF)||1:50 -1:200|
|Immunoprecipitation (IP)||2 µg|
|Western Blot (WB)||1:100 - 1:1000|
|Western Blot (WB)||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Chemical|
|Published species reactivity||Mouse Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||O-phospho-L-tyrosine and O-phospho-DL-tyramine|
|Storage buffer||PBS with 40% glycerol|
|Contains||0.05% sodium azide|
|Storage conditions||4° C|
MA4-003 detects phosphotyrosine from most species and tissues.
MA4-003 has been successfully used in ELISA, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and FACS procedures. This antibody detects proteins which contain phosphorylated tyrosine residues.
The MA4-003 antigen is O-phospho-L-tyrosine and O-phospho-DL-tyramine.
The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Phosphotyrosine; pTyr; pY
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