|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:5000|
|Western Blot (WB)||See 4 publications below|
|Immunoprecipitation (IP)||See 2 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 2 publications below|
|Tested Species reactivity||Chemical|
|Published species reactivity||Rat , Non-human primate , Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Phosphotyrosine coupled to carrier protien.|
|Storage buffer||PBS, pH 7.5, with 50% glycerol|
|Contains||3mM sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
Protein kinases like platelet-derived growth factor and epidermal growth factor have the ability to phosphorylate tyrosine residues of other proteins like GTPase activating protein. Protein kinases play a prominent role in the regulation of normal growth as well as tumor growth. A number of retroviruses encode proteins with tyrosine kinase activity and infection with such a virus is associated with a 10-20 fold increase in phosphotyrosine on the virally encoded protein as well as on other cellular proteins. This antibody, from clone 1G2, is capable of specifically binding to proteins which contain phosphorylated tyrosine residues, but will not react with any other phosphorylated amino acids, proteins or nuecleotides, and is excellent for the study of growth factor receptors, oncogenes, and signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Phosphotyrosine; pTyr; pY
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