|Flow Cytometry (Flow)||1/200 - 1/400|
|Immunofluorescence (IF)||1/200 - 1/1000|
|Immunohistochemistry (IHC)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
|Tested Species reactivity||Human, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human Pirh2 expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA5-15596 targets Pirh2 in FACS, IF, IHC, and WB applications and shows reactivity with Human and Rat samples.
The MA5-15596 immunogen is purified recombinant fragment of human Pirh2 expressed in E. Coli.
MA5-15596 detects Pirh2 which has a predicted molecular weight of approximately 30kDa; 60kDa (homodimer).
The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including TP53, HDAC1 and CDKN1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Androgen receptor N-terminal-interacting protein; androgen-receptor N-terminal-interacting protein; ARNIP; CH-rich interacting match with PLAG1; CH-rich-interacting match with PLAG1; CHIMP; DKFZp586C1620; E3 ubiquitin-protein ligase Pirh2; hARNIP; hPirh2; p53-induced protein with a RING-H2 domain; p53-induced RING-H2 protein; PIRH2; PRO1996; RCHY1; ring finger and CHY zinc finger domain containing 1, E3 ubiquitin protein ligase; RING finger and CHY zinc finger domain-containing protein 1; RING finger protein 199; RING-type E3 ubiquitin transferase RCHY1; RNF199; Zinc finger protein 363; zinc finger, CHY-type; ZNF363
Gene Aliases: ARNIP; CHIMP; PIRH2; PRO1996; RCHY1; RNF199; ZCHY; ZNF363
UniProt ID: (Human) Q96PM5
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