|ELISA (ELISA)||Assay Dependent|
|Immunoelectrophoresis (IE)||Assay Dependent|
|Tested Species reactivity||Human|
|Host / Isotype||Sheep / IgG|
|Immunogen||Plasminogen from human plasma.|
|Storage buffer||0.01M HEPES, pH 7.4, with 0.15M NaCl, 50% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-74143 detects Plasminogen from human samples.
PA1-74143 has been successfully used in ELISA and immunoelectrophoresis applications.
The PA1-74143 immunogen is Plasminogen from human plasma.
Plasminogen (Pg) is synthesized in the liver and circulates in plasma at a concentration of ~200 µg/ml (~2.3 µM). Plasminogen is a single-chain glycoprotein of ~88 kDa that consists of a catalytic domain followed by five kringle structures. Within these kringle structures are four low-affinity lysine binding sites and one high-affinity lysine binding site. It is through these lysine binding sites that plasminogen binds to fibrin and to a2antiplasmin. Native plasminogen (gluplasminogen) exists in two variants that differ in their extent of glycosylation, and each variant has up to six isoelectric forms with respect to sialic acid content, for a total of 12 molecular forms. Activation of glu-plasminogen by the plasminogen activators urokinase (UPA), or tissue plasminogen activator (tPA) occurs by cleavage after residue Arg560 to produce the two-chain active serine protease plasmin. In a positive feedback reaction, the plasmin generated cleaves an ~8 kDa peptide from glu-plasminogen, producing lys77-plasminogen which has a higher affinity for fibrin and when bound is a preferred substrate for plasminogen activators such as urokinase. Additional activators of plasminogen include kallikrein and activated factor XII. The primary inhibitor of plasmin in plasma is a2antiplasmin. Other physiological inhibitors of plasmin include a2macroglobulin and antithrombin.
Protein Aliases: plasmin; plasminogen
Gene Aliases: RP1-81D8.1
UniProt ID: (Human) P00747
Entrez Gene ID: (Human) 5340