|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunoprecipitation (IP)||2 ug/ml|
|Western Blot (WB)||1-3 µg/ml|
|Immunocytochemistry (ICC)||See 3 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Western Blot (WB)||See 3 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human , Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human recombinant L-plastin protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-11921 targets Plastin-L in IF, IP, and WB applications and shows reactivity with Human samples.
The MA5-11921 immunogen is human recombinant L-plastin protein.
L-plastin is a leukocyte specific actin bundling protein from fimbrin family of proteins. It has two actin-binding domains and two EF hand type calcium-binding domains. It gets phosphorylated on the serine residue in its headpiece region in response to a variety of inflammatory stimuli. It is hypothesized that L-plastin may play a role in regulating integrin mediated adhesion in leukocytes. L-plastin is expressed in majority of human cancer cell lines derived from solid tumors. Its expression is positively upregulated by testosteronse in andrgen receptor positive prostrate and breast cancer cells through three androgen receptor binding elements located in the upstream region of the gene.
Protein Aliases: bA139H14.1 (lymphocyte cytosolic protein 1 (L-plastin)); epididymis secretory protein Li 37; L-plastin; L-plastin (Lymphocyte cytosolic protein 1) (LCP-1) (LC64P); LC64P; LCP-1; Lymphocyte cytosolic protein 1; lymphocyte cytosolic protein 1 (L-plastin); Lymphocyte cytosolic protein-1 (plasmin); plastin 2; Plastin-2
Gene Aliases: CP64; HEL-S-37; L-PLASTIN; LC64P; LCP1; LPL; PLS2
UniProt ID: (Human) P13796
Entrez Gene ID: (Human) 3936