|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||3-5 µg/ml|
|Western Blot (WB)||3-5 µg/ml|
|ELISA (ELISA)||See 1 publications below|
|Immunohistochemistry (IHC)||See 3 publications below|
|Tested Species reactivity||Bovine, Deer, Human, Sheep|
|Published species reactivity||Hamster , Sheep Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to residues S(146) R P L I H F G S D Y E D R(159) of bovine PrP.|
|Storage buffer||1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-750 detects prion protein (PrP) protein from a variety of species that have the conserved n-IHFG-c epitope, including agriculturally important animal species such as sheep, bovine, deer, and elk. MA1-750 has been successfully used in Western blot, immunohistochemistry and ELISA procedures. By Western blot, this antibody detects a 33-35 kDa protein from normal animals and a 27-30 kDa protein which represents PrP in brain protease treated tissue extracts from infected animals. Immunohistochemical staining of PrP(Sc) in infected sheep brain with MA1-750 results in intense staining of the spongiform lesions. In immunohistochemical procedures this antibody detects only the PrP(Sc) and not PrP(C). The MA1-750 immunogen is a synthetic peptide corresponding to residues S(146) R P L I H F G S D Y E D R(159) of bovine PrP. This peptide (Cat. # PEP-051) is available for use in neutralization and control experiments. MA1-750 is known to specifically recognize a conserved epitope of the PrP(Sc) protein comprising the amino acids n-IHFG-c.
Cellular prion protein (PrPC) is a highly conserved, 33-35 kDa protease-sensitive, sialoglycoprotein of unknown function which is endogenously expressed in brain. Studies have shown this protein to be anchored to the external surface of the cell membrane by glycosyl phosphatidylinositol. A post-translationally altered, protease-resistant PrPC isoform ( PrP-Scrapie or PrPSc) of 27-30 kDa is found in association with several fatal neurodegenerative disorders collectively termed transmissible spongiform encephalopathies (TSEs). Deposition of PrPSc in the central nervous system is a reliable indication of a TSE. Prion diseases include scrapie in sheep and goats, bovine spongiform encephalopathy or "Mad Cow disease", and chronic wasting disease (CWD) in deer and elk. PrP is responsible for Creutzfeldt-Jakob disease (CJD), Gerstmann-Straussler-Scheinker (GSS) disease, Fatal Familial Insomnia (FFI), and Kuru in humans.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Alternative prion protein; AltPrP; ASCR; CD230; CD230 antigen; CJD; GSS; Major prion protein; Major scrapie-associated fibril protein 1; MGC26679; prion protein (p27-30) (Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia); prion protein precursor PrP; prion protein PrP; prion protein variant a; prion protein variant b; prion-related protein; PRNP; PrP; PrP27-30; PrP33-35C; PrPc
Gene Aliases: ALTPRP; ASCR; BOS_13027; CD230; CJD; GSS; KURU; p27-30; PRIP; PRNP; PRP; PrP27-30; PrP33-35C; PRPC; SIP
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