|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 23 and 272 of Human Prohibitin|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% Proclin 300|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-27329 targets Prohibitin in IF, IHC (P), and WB applications and shows reactivity with Human, mouse, and Rat samples.
The PA5-27329 immunogen is recombinant fragment corresponding to a region within amino acids 23 and 272 of Human Prohibitin.
Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B-cell receptor-associated protein 32; BAP 32; epididymis luminal protein 215; epididymis secretory sperm binding protein Li 54e; PHB1; Prohibitin
Gene Aliases: Bap32; HEL-215; HEL-S-54e; PHB; PHB1
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.