|Flow Cytometry (Flow)||Assay dependent|
|Immunocytochemistry (ICC)||5 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1 µg/ml|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunocytochemistry (ICC)||See 5 publications below|
|Western Blot (WB)||See 12 publications below|
|Immunohistochemistry (IHC)||See 9 publications below|
|Immunoprecipitation (IP)||See 2 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
|Tested Species reactivity||Horse, Human, Pig, Rabbit, Rat|
|Published species reactivity||Rabbit , Rat , Non-human primate , Bovine , Hamster , Human Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified rat liver PRL receptor.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-610 detects prolactin (PRL) receptor from rat, human, rabbit, horse, and pig tissues.
MA1-610 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunofluorescence, and FACS procedures. By Western blot, this antibody detects an ~40 kDa protein and an ~100 kDa protein representing both the short and long forms of the PRL receptor, respectively, in various tissues. Immunohistochemical staining of PRL receptor in rat Islets of Langerhans with MA1-610 results in a staining pattern consistent with cytoplasmic vesicle staining. MA1-610, after biotinylation, has been used to detect human lymphocyte PRL receptor by FACS. MA1-610 does not inhibit the interaction of prolactin with the receptor.
The MA1-610 immunogen is purified PRL receptor from rat liver. This antibody has been shown to react with the extracellular portion of the receptor, but not in the ligand binding domain. MA1-610 does not inhibit the interaction of prolactin with the receptor.
Reconstitute with 100 ul PBS.
Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa.
The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: hPRL receptor; Lactogen receptor; Prl R; PRL-R; Prolactin receptor; prolactin receptor short isoform; secreted prolactin binding protein
Gene Aliases: HPRL; hPRLrI; MFAB; PRLR; RATPRLR
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