|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 238 of Human Proteasome 20S alpha 1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-28030 targets Proteasome 20S alpha 1 in IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples.
The PA5-28030 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 238 of Human Proteasome 20S alpha 1.
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 1; 20S proteasome subunit C2; 30 kDa prosomal protein; alpha-type; epididymis secretory protein Li 275; HC2; Macropain subunit C2; macropain subunit nu; Multicatalytic endopeptidase complex subunit C2; NU; p; PROS-30; PROS30; proteasome (prosome, macropain) subunit, alpha type, 1; Proteasome component C2; Proteasome nu chain; proteasome subunit; Proteasome subunit alpha type-1; proteasome subunit nu; proteasome subunit, alpha-type, 1; protein P30-33K; testicular tissue protein Li 150
Gene Aliases: alpha-type; C2; HC2; HEL-S-275; NU; Pros-30; PROS30; PSC2; PSMA1
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