|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:250|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Bovine, Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues T(1620) K L P K K R K R D P G S V G(1634) of human PA200.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-1961 detects PA200 from bovine, human, mouse and rat samples.
PA1-1961 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~200 kDa protein representing PA200 from bovine testis tissue extract. In mouse tissues, this antibody detects both a 200 kDa and a 160 kDa protein. In mouse tissues, the 200 kDa species is most abundant in testis whereas the 160 kDa species is enriched in the spleen. Immunofluorescence staining of PA200 in HeLa cells results in diffuse nuclear staining prior to ionizing radiation treatment. Immunofluorescence staining of PA200 in HeLa cells after ionizing radiation treatment results in focal aggregate staining within the nucleus.
The PA1-1961 immunogen is a synthetic peptide corresponding to residues T(1620) K L P K K R K R D P G S V G(1634) of human PA200.
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Two transcripts encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: proteasome (prosome, macropain) activator subunit 4; Proteasome Activator 200 kDa; Proteasome activator complex subunit 4; Proteasome activator PA200; Protein TEMO
Gene Aliases: AA409398; AU041366; BOS_11598; KIAA0077; mKIAA0077; PA200; PSME4; TEMO
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