|ELISA (ELISA)||1 ug/mL|
|Western Blot (WB)||1 ug/mL|
|Tested Species reactivity||Yeast|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide from the amino terminus of yeast Pst1|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
PA5-34414 can be used with blocking peptide PEP-1456.
Protoplast secreted protein 1 (Pst1) is an anchored plasma membrane protein. Pst1 was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction. Pst1 is a protein with 444 amino acids and is attached to yeast cell wall via a glycosylphosphatidylinisotol (GPI) anchor. Pst1 contains 15 potential N-linked glycosylation sites and is heavily glycosylated. It migrates at approximately 200 kDa on SDS-PAGE when produced in wild type S. cerevisiae. Our Pst1 protein was expressed in a genetically manipulated triple-mutant (TM) S. cerevisiae stain, which results in the production of sole Man8GlcNAc2 carbohydrate structures and Pst1 migration at approximately 100 kDa. When produced in the TM yeast, Pst1 can be recognized by several glycan-specific HIV-1 broadly neutralizing antibodies, including 2G12 and recently identified PGT antibodies. Among several heavily N-glycosylated TM yeast glycoproteins, Pst1 shows higher affinity for 2G12 and efficiently inhibits gp120 interactions with 2G12 and DC-SIGN, and it also blocks 2G12-mediated neutralization of HIV-1 pseudoviruses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cell wall mannoprotein PST1; Haze protective factor 2; Protoplast secreted protein 1; Pst1; Pst1p
Gene Aliases: D4214; HPF2; PST1; YD9609.09; YDR055W
UniProt ID: (Baker's yeast) Q12355
Entrez Gene ID: (Baker's yeast) 851625
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