|Immunomicroscopy (IM)||Assay Dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000-1:5000|
|Immunocytochemistry (ICC)||See 4 publications below|
|Immunohistochemistry (IHC)||See 3 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
|Western Blot (WB)||See 3 publications below|
|Tested Species reactivity||Bovine, Dog, Hamster, Human, Non-human primate, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant canine rab 9a.|
|Storage buffer||ascites diluted in PBS|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA3-067 recognizes prenylated and non-prenylated rab 9 from canine, human, monkey, rat, hamster and bovine tissues. This antibody does not cross-react with other rab family members.
MA3-067 has been successfully used in Western blot, immunofluorescence, EM immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~26 kDa protein representing rab 9 from K562 cell extract.
The MA3-067 antigen is recombinant canine rab 9a.
Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components.
It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: rab-9A; rab9 GTP-binding protein; RAB9, member RAS oncogene family; Ras-related protein Rab-9; Ras-related protein Rab-9A
Gene Aliases: BOS_26348; RAB9; RAB9A
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.