|Immunohistochemistry (Frozen) (IHC (F))||10 µg/ml|
|Immunoprecipitation (IP)||4 µg|
|Western Blot (WB)||0.5-2 µg/ml|
|Immunocytochemistry (ICC)||See 1 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant, full length, human RAC protein.|
|Storage buffer||0.1M tris glycine, pH 7.4, with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA1-20580 detects RAC in human, mouse, and rat samples.
MA1-20580 has been successfully used in immunohistochemistry (frozen), immunoprecipitation, and Western blot procedures. Detects a band of approximately 21 kDa.
The MA1-20580 immunogen corresponds to recombinant, full length, human RAC protein.
Store at 4°C short term. For extended storage, aliquot and store at -20°C, avoiding freeze/thaw cycles.
RAC, a small GTPase in the ras family, regulates the formation of membrane ruffles, lamellipodia and filopodia. It is involved in cytoskeletal actin organization and transformation. The p21 activated kinases (PAKs) are direct targets of active Rac and Cdc42 which can induce the assembly of polarized cytoskeletal structures when expressed in fibroblasts. RAC1 and RAC2 are also essential components of NADPH oxidase, the enzyme responsible for generating free radicals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AKT; CYK4; Male germ cell RacGap; MGC99656; MgcRacGAP; PKB-ALPHA; PRKBA; Protein CYK4 homolog; RAC; Rac GTPase-activating protein 1; RAC-ALPHA; racgap1
Gene Aliases: AI227039; AI327394; Band25; CYK4; gtl11; GTPase; HsCYK-4; ID-GAP; KIAA1478; MGCRACGAP; mKIAA1478; RACGAP1
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