|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Yeast|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 187 of Yeast RAD3|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-32174 targets RAD3 in WB applications and shows reactivity with Yeast samples.
The PA5-32174 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 187 of Yeast RAD3.
ATP-dependent DNA helicase involved in excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Necessary for excision of pyrimidine dimers. Also unwinds DNA/RNA duplexes. Plays an essential role in the cell viability. Involved in the maintenance of the fidelity of DNA replication. Acts as component of the general transcription and DNA repair factor IIH (TFIIH) core, which is essential for both basal and activated transcription, and is involved in nucleotide excision repair (NER) of damaged DNA. TFIIH has CTD kinase and DNA-dependent ATPase activity, and is essential for polymerase II transcription in vitro.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DNA repair helicase RAD3; General transcription and DNA repair factor IIH subunit RAD3; Rad3p; TFIIH subunit RAD3
Gene Aliases: RAD3; REM1; YER171W
UniProt ID: (Baker's yeast) P06839
Entrez Gene ID: (Baker's yeast) 856918
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