|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 1 and 44 of Human RASGRP3|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-28091 targets RASGRP3 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-28091 immunogen is synthetic peptide corresponding to a region within amino acids 1 and 44 of Human RASGRP3.
Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RASGRP3, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Calcium and DAG-regulated guanine nucleotide exchange factor III; calcium- and diacylglycerol-regulated guanine nucleotide exchange factor III; CalDAG-GEFIII; GRP3; Guanine nucleotide exchange factor for Rap1; RAS guanyl releasing protein 3 (calcium and DAG-regulated); Ras guanyl-releasing protein 3
Gene Aliases: GRP3; KIAA0846; RASGRP3
UniProt ID: (Human) Q8IV61
Entrez Gene ID: (Human) 25780
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.