|Immunocytochemistry (ICC)||2.5 µg/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||0.5 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 16 amino acid peptide near the center of human RGS21.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
A suggested positive control is HepG2 cell lysate.
PA5-20760 can be used with blocking peptide PEP-0874.
Regulator of G-protein signaling (RGS) proteins contain an 120 amino acid conserved domain, termed the RGS domain, that acts as a GTPase-activating protein that acts to reduce the signal transmitted by the receptor-activated G-alpha subunit. RGS21 is a recently identified member of this family that has been reported to be selectively expressed in subpopulations of taste bud cells and co-expressed with bitter and sweet transduction components such as alpha-gusticin, phospholipase Cbeta2, T1R2/T1R3 sweet taste receptors and T2R bitter taste receptors. Other reports indicate that RGS21 is more widely expressed. Binding assays demonstrate that RGS21 binds alpha-gusticin in a conformation-dependent manner and may do the same with T1R receptors, suggesting that RGS21 may play a role in sweet and bitter taste transduction processes.
Protein Aliases: Regulator of G-protein signaling 21; regulator of G-protein signalling 21; RGS21
Gene Aliases: RGS21
UniProt ID: (Human) Q2M5E4
Entrez Gene ID: (Human) 431704