|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||2 µg/ml|
|Western Blot (WB)||See 1 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Rat Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues G(718) N P T K G R V K K K E K T P L R D(735) of human RIP140.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-841 detects recombinant human Receptor-Interacting Protein 140 (RIP 140).
PA1-841 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects two proteins at ~140 kDa representing human RIP 140 expressed in rabbit reticulocyte lysate.
The PA1-841 immunogen is a synthetic peptide corresponding to residues G(718) N P T K G R V K K K E K T P L R D(735) of human RIP140. This immunizing peptide (Cat. # PEP-030) is available for use in neutralization and control experiments.
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate.
Receptor-Interacting Protein 140 (RIP140) has been shown to enhance estrogen receptor transcriptional activity through the AF2 activation domain in an agonist dependent manner and has also been implicated to be involved in other steroid receptor pathways, including the retinoid pathway. RIP140 has recently been shown to interact with peroxisome proliferator-activated receptor-alpha (PPAR alpha) and has been proposed to competitively inhibit PPAR alpha interaction with Steroid Receptor Coactivator-1 (SRC-1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ERAP 140; Nuclear factor RIP140; Nuclear receptor-interacting protein 1; Receptor-Interacting Protein 140
Gene Aliases: NRIP1; RIP140
UniProt ID: (Human) P48552
Entrez Gene ID: (Human) 8204
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