|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Immunohistochemistry (IHC)||5 ug/ml|
|Western Blot (WB)||1 µg/ml|
|Tested Species reactivity||Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 473 to 486 of murine RIP3.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
In Western blot applications, this antibody detects a band at ~57kDa. A suggested positive control is NIH-3T3 cell lysate.
PA5-19956 can be used with blocking peptide PEP-0081.
Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-kappa-B activation. Overexpression of RIP3 induces apoptosis and NF-kappa-B activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Homocysteine respondent protein HCYP2; mRIP3; receptor interacting protein 3; Receptor-interacting protein 3; Receptor-interacting serine/threonine-protein kinase 3; RIP-3; RIP-like protein kinase 3
Gene Aliases: 2610528K09Rik; AW107945; Hcyp2; Rip3; Ripk3
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