|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from the C-terminal region of human POLR1B|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.4, with 150mM NaCl, 50% glycerol|
|Contains||0.02% sodium azide|
Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: A127; DNA-directed RNA polymerase I 135 kDa polypeptide; DNA-directed RNA polymerase I 135kDa polypeptide; DNA-directed RNA polymerase I subunit RPA2; polymerase (RNA) I polypeptide B; polymerase (RNA) I polypeptide B, 128kDa; polymerase (RNA) I subunit B; RNA polymerase 1-2; RNA polymerase 1-2, 128 kDa subunit; RNA polymerase I (127 kDa subunit); RNA polymerase I polypeptide B; RNA polymerase I subunit 2; RPA135
Gene Aliases: 128kDa; D630020H17Rik; POLR1B; RPA116; RPA135; RPA2; Rpo1-2
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.