|ChIP assay (ChIP)||1-10 ul|
|ELISA (ELISA)||1:100 - 1:2000|
|Immunocytochemistry (ICC)||1:50 - 1:100|
|Immunofluorescence (IF)||1:50 - 1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Immunoprecipitation (IP)||1:10 - 500|
|Western Blot (WB)||1:1000 - 1:20000|
|ChIP assay (ChIP)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Yeast|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Chemically synthesized phospho-ser 5 YSPTSpPS (Human).|
MA1-46093 reacts with DNA-directed RNA Polymerase II Largest Subunit in human, yeast and mouse samples. Expected to cross-react with Drosophilia melanogaster, hamster, S. pombe, and Arabidopsis thaliana due to sequence homology.
MA1-46093 has been successfully used in Western Blot, Chromatin Immunoprecipitation, ELISA, Immunocytochemistry/Immunofluorescence, IHC (P) and Immunoprecipitation applications.
The MA1-46093 immunogen is 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). This antibody detects both the phosphorylated and non-phosphorylated forms.
The POLR2A gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII. CTD4H8 can be used as a positive control in chromatin immunoprecipitation (ChIP) assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DNA-Directed RNA Polymerase II Largest Subunit; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-Directed RNA Polymerase II Subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 126.96.36.199; HRPB220; POLR2; POLR2A; POLRA; polymerase (RNA) II (DNA directed) polypeptide A (220kDa); polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; RNA polymerase II 1; RNA Polymerase II Subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RpIILS; RPOL2
Gene Aliases: 220kDa; hRPB220; hsRPB1; POLR2; POLR2A; POLRA; RPB1; RPBh1; Rpii215; RpIILS; RPO2; Rpo2-1; RPOL2
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