|Gel Shift (GS)||1mg/mL|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
|Immunocytochemistry (ICC)||See 2 publications below|
|Western Blot (WB)||See 3 publications below|
|ELISA (ELISA)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Mouse Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Clone||2H10 (70C or alphaSSB70C)|
|Immunogen||Purified human RPA protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-13953 targets RPA/p70 in GS, IP, and WB applications and shows reactivity with Human samples.
The MA5-13953 immunogen is purified human RPA protein.
Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB) is involved in DNA replication, repair, and recombination. RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase alpha and delta. RPA70 is composed of three domains: an N-terminal domain that is not required for ssDNA binding or SV40 replication, a central DNA-binding domain, and a C-terminal domain that is essential for subunit interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: MSTP075; Replication factor A protein 1; Replication protein A 70 kDa DNA-binding subunit; replication protein A1, 70kDa; RF-A protein 1; RP-A p70; Single-stranded DNA-binding protein
Gene Aliases: HSSB; MST075; REPA1; RF-A; RP-A; RPA1; RPA70
UniProt ID: (Human) P27694
Entrez Gene ID: (Human) 6117
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