|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 239 and 566 of Human RPA70|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-29616 targets RPA70 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-29616 immunogen is recombinant fragment corresponding to a region within amino acids 239 and 566 of Human RPA70.
Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: HSSB; MST075; MSTP075; REPA1; Replication factor A protein 1; Replication protein A 70 kDa DNA-binding subunit; replication protein A1, 70kDa; RF-A protein 1; RP-A; RP-A p70; RPA70; Single-stranded DNA-binding protein
Gene Aliases: HSSB; MST075; REPA1; RF-A; RP-A; RPA1; RPA70
UniProt ID: (Human) P27694
Entrez Gene ID: (Human) 6117
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