|Western Blot (WB)||1-3 µg/ml|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 174–188 of human Runx1|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with bovine and non-human primate based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Runx1 belongs to the mammalian RUNX family of DNA binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. Runx1 is essential for hematopoietic stem cell generation in the vascular regions of the aorta, vitelline and umbilical arteries, yolk sac and placenta. Runx1 is expressed in all tissues except brain and heart, with highest levels in thymus, bone marrow and peripheral blood.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Acute myeloid leukemia 1 protein; alpha subunit 2; aml1 oncogene; AML1-EVI-1; AML1-EVI-1 fusion protein; AMLCR1; CBF-alpha-2; CBFA2; core binding factor alpha 2; core-binding factor; Core-binding factor subunit alpha-2; core-binding factor, runt domain, alpha subunit 2; Oncogene AML-1; PEA2-alpha B; PEBP2-alpha B; PEBP2aB; Polyomavirus enhancer-binding protein 2 alpha B subunit; runt domain; runt domain, alpha subunit 2; Runt-related transcription factor 1; RUNX1/ZNF687 fusion; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; transcription factor
Gene Aliases: AML1; AML1-EVI-1; AMLCR1; CBF-alpha-2; CBF2alpha; CBFA2; EVI-1; Pebp2a2; PEBP2aB; PEBP2alpha; Pebpa2b; RUNX1
UniProt ID: (Human) Q01196
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