|Western Blot (WB)||2-5 µg/ml|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues A(231) F N P Q P Q S Q M Q D T R Q(241) of human AML-1/ETO.|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
Suggested positive control: antigen standard for RUNX1 (transient overexpression lysate), Ramos, Raw.
AML-1 is a DNA-binding protein which specifically interacts with a sequence belonging to the group of enhancer core motifs, TGT/cGGT. The AML1 gene on chromosome 21 is disrupted in the (8;21)(q22;q22) translocation associated with acute myelogenous leukemia and encodes a protein with a central 118-amino-acid domain with 69% homology to the Drosophila pair-rule gene, runt. AML-1 translocation is frequently observed in acute myeloid leukemia. It is a key transactivator of tissue-specific genes of the hematopoietic and bone lineages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Acute myeloid leukemia 1 protein; AML-1; AML1-EVI-1 fusion protein; CBF-alpha-2; core binding factor alpha 2; Core-binding factor subunit alpha-2; core-binding factor, runt domain, alpha subunit 2; Oncogene AML-1; PEA2-alpha B; PEBP2-alpha B; Polyomavirus enhancer-binding protein 2 alpha B subunit; runt domain, alpha subunit 2; Runt-related transcription factor 1; RUNX1/ZNF687 fusion; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; transcription factor
Gene Aliases: AML1; AML1-EVI-1; AMLCR1; CBF-alpha-2; CBF2alpha; CBFA2; EVI-1; Pebp2a2; PEBP2aB; PEBP2alpha; Pebpa2b; RUNX1
UniProt ID: (Human) Q01196
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.