|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:100-1:500|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH, corresponding to amino acids 576-592 of Human RYK.|
|Contains||0.09% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
PA1-30713 detects RYK from human samples.
PA1-30713 has been successfully used in immunohistochemistry (frozen tissue), immunohistochemistry (paraffin tissue), and Western blot applications. A suggested positive control for this product is Jurkat cell lysate.
The PA1-30713 immunogen is Synthetic peptide conjugated to KLH, corresponding to amino acids 576-592 of Human RYK.
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: D3S3195; ERK3; Hydroxyaryl-protein kinase; JTK5A protein tyrosine kinase; Receptor-like tyrosine kinase; RYK1; Tyrosine-protein kinase RYK
Gene Aliases: D3S3195; JTK5; JTK5A; RYK; RYK1
UniProt ID: (Human) P34925
Entrez Gene ID: (Human) 6259
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