|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 12 amino acid synthetic peptide derived from the extreme carboxy-terminus of the human Raf-1 protein. This sequence is 100% conserved in mouse and rat Raf-1 proteins.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: C-RAF; C-Raf proto-oncogene, serine/threonine kinase; CRAF; Craf1 transforming; EC 184.108.40.206; kinase Raf1; murine leukemia viral (v-raf-1) oncogene homolog 1 (3611-MSV); murine sarcoma 3611 oncogene 1; Oncogene RAF1; Protein kinase raf 1; Proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; Raf-1; RAF1; sarcoma 3611 oncogene; Similar to murine leukemia viral (V-raf-1) oncogene homolog 1; v-raf-1 leukemia viral oncogene 1; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1
Gene Aliases: 6430402F14Rik; AA990557; BB129353; c-Raf; CMD1NN; CRAF; Craf1; D830050J10Rik; NS5; RAF; Raf-1; RAF1; v-Raf
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.