|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Western Blot (WB)||1:5,000-1:20,000|
|Tested Species reactivity||Rat|
|Host / Isotype||Chicken / IgY|
|Immunogen||Rat IgG whole molecule|
|Storage buffer||0.05M tris HCl, pH 7.2, with 0.15M NaCl, 10mg/ml BSA, 0.001M MgCl2, 0.0001M ZnCl2, 50% glycerol|
|Contains||0.01% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
PA1-30741 detects Rat IgG from rat samples.
PA1-30741 has been successfully used in ELISA, immunohistochemistry (frozen tissue), Western blot, immunohistochemistry (paraffin tissue) applications.
The PA1-30741 immunogen is: Rat IgG whole molecule
Thermo Scientific Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.