|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||1.0 µg/ml|
|Western Blot (WB)||0.5 µg/ml|
|Western Blot (WB)||See 2 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) A S K E M F E D T V E E(13) C of human RbAp46.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-868 detects retinoblastoma protein (Rb) associated protein 46 (RbAp46) from human cells.
PA1-868 has been successfully used in IF, Western blot and immunocytochemical procedures. By Western blot, this antibody detects an ~46 kDa protein representing RbAp46 from HeLa cell lysate. Immunocytochemical staining of RbAp46 in HeLa cells with PA1-868 results in nuclear staining.
The PA1-868 immunogen is a synthetic peptide corresponding to residues M(1) A S K E M F E D T V E E(13) C of human RbAp46. This sequence is completely conserved between human and mouse. This immunizing peptide (Cat. # PEP-083) is available for use in neutralization and control experiments.
Retinoblastoma protein (Rb) associated protein 46 (RbAp46) is a nuclear protein that has been shown to interact directly with retinoblastoma protein and is a component of the mSin3 corepressor complex as are the histone deacetylase proteins (HDACs). RbAp46 shares about 75% sequence identity with the closely related RbAp48, and appears to be functionally related to the yeast MSI1 protein in that overexpression of RbAp46 suppresses heat shock sensitivity in a RAS pathway dependent manner. RbAp46 has also been demonstrated to be up-regulated by the Wilms and quote; tumor suppressor gene product, WT1. Additionally, cells transfected with RbAp46 have shown strong inhibition of growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: G1/S transition control protein-binding protein RbAp46; Histone acetyltransferase type B subunit 2; Histone-binding protein RBBP7; Nucleosome-remodeling factor subunit RBAP46; RBBP-7; Retinoblastoma Associated Protein 48; retinoblastoma binding protein 7; Retinoblastoma-binding protein 7; Retinoblastoma-binding protein p46; retinoblastoma-binding protein RbAp46
Gene Aliases: RBAP46; RBBP7
UniProt ID: (Human) Q16576
Entrez Gene ID: (Human) 5931
Molecular Function: receptor
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